Halo Yohans*
This review mainly focused on methods that are used for identifying novel bacterial antigens that can use in recombinant subunit vaccine development. Particularly, it describes the processes involved in reverse vaccinology, genomic function, bioinformatics and immunological approaches and some associated complementary technologies such as proteomics that can be used in identification of new and potentially useful vaccine antigens. Results obtained from the application of these methods are forming basis for a new generation of vaccine for use in the control of bacterial infections of humans and animals.