జర్నల్ ఆఫ్ ప్లాంట్ పాథాలజీ & మైక్రోబయాలజీ

నైరూప్య

Polymerase and Coat Protein Gen Were Targeted and Characterized in Wheat Yellow Dwarf Virus, Amino acids Deduced was Compared With Group Members in Genbank

Hoda Waziri*

Wheat plants grown in Egypt shows virus like symptoms Wheat yellow dwarf has been characterized using RT-PCR technique. Two coding regions of Wheat yellow dwarf virus (WYDV- PAV) isolate for the polymerase gene (P1) located in Open Reading Frame (ORF1) and coat protein gene located in Open Reading Frame (ORF3) were targeted. Two
sets of specific primers were designed according to of BYDV-PAV isolate in Genbank. The DNA fragments of ORF1and ORF3 of an Egyptian isolate of BYDV-PAV were cloned and sequenced. The sequence contained a fulllength ORF1 coding for the viral polymerase gene. It comprises 910 nt in length encodes a predicted polypeptide chain of 303 amino acids with a M(r) of 34.67. On the other hand, the sequence data for the ORF3 coding for the viral coat protein revealed that it was 603 bp in length encodes a predicted protein 200 amino acids, with molecular weight of 21.96 KDa. However, the phylogenetic homology tree based on the multiple sequence alignments of the Egyptian isolate Egy-Wz with those isolates available in NCBI GenBank revealed that the polymerase gene shared 76.5%-99% and 71.6%-93.2% sequence identities at amino acid and nucleotide levels with the 05GG2, PAV 014 and PAV014, PAV-Aus isolates respectively. On the other hand, the coat protein gene showed 85.1% -99.5% and 89.7%-99.2% sequence similarity with two isolates 06KM14 and 05GG2 at the amino acid and nucleotide level, respectively.