క్లినికల్ & మెడికల్ బయోకెమిస్ట్రీ

నైరూప్య

Molecular and Morphological Identification of Fish Gut Microbiota at Different Growth Stage of Labeo rohita

Muhammad Waqar Mazhar, Ahmad Raza, Selinay Başak Erdemli Köse, Hira Iftikhar, Hira Tahir, He-Ming Zhou, Mudasara Sikandar,Tsvetina Nikolova, Eisha Javed

Introduction: The gut micro-biota plays a significant role in the fish health and pathogenicity. L.rohita is well known farmed fish it also culture with other major carps.

Methodology: The present study was conducted to determine the molecular and morphological identification of gut micro-biota of L.rohita collected from the fish hatchery Faisalabad. Isolation of bacteria was carried out through the culture techniques. The morphological identification of bacterial isolates was determined by the gram staining, biochemical tests TSI and MR-VP test. The molecular identification was done by 16S rRNA techniques.

Results: The results of present research work was showed that the 20 strains of bacterial were isolated from the gut of L.rohita and all these isolates were gram negative and morphological identify on the basis of biochemical tests i.e. TSI and MR-VP test. Biofilm result indicated that six, eight and six isolates showed the weak, moderate and strong biofilm.

Conclusion: The highest and lowest resistance showed against cefadroxil and Levofloxin respectively. The lowest intermediate isolates were found against cefadroxil, polymyxin B and colistin and highest intermediate were found against ceftriaxone. The highest sensitive isolates were found against Levofloxin and lowest sensitive isolate were found in cefadroxil, nitrofurantoin and cefoxitin. On the basis of 16Sr RNA sequencing and phylogenetic relationship among isolates 17 isolate were determined in the present study. Klebsiella pneumonia, Enterobacter cloacae, Pseudomonas oleovorans, Morganella morganii, Citrobacter freundii, Proteus mirabilis, Citrobacter braakii, Enterobacter hormaechei, Psychrobacter sanguinis, Shigella dysenteriae, Citrobacter cronae, Shigella sonnei, Pseudomonas sihuiensis, Proteus mirabilis, Enterobacter hormaechei were found in the intestine of L.rohita.