జర్నల్ ఆఫ్ ప్లాంట్ పాథాలజీ & మైక్రోబయాలజీ

నైరూప్య

Management of Collar Rot Disease Caused by Macrophomina phaseolina in Jatropha curcas - Green Diesel Plant

Aneesha Singh, Prakruti Patel and Pradeep Kumar Agrawal

Physic nut (Jatropha curcas) has become popular due to its potential for biodiesel production. Intensified cultivation of J. curcas has raised the importance of plant disease management. Collar rot disease has been reported in J. curcas as a major cause of severe plant damage. In the present study, we report the management of coller rot diseaseusing fungicides and plant extracts. Two fungicides were tested in vitro for antifungal activity using well diffusion method. Carbemedozo and Macrinite (CM-75) was found to be most effective at 0.1% to inhibit mycelial growth and it developed largest inhibition zone of 50 mm and 0.1% Bavistin (BV) developed inhibition zone of 47 mm. The complete inhibition of the fungal growth was achieved at 0.1% CM-75 and 0.2% BV. Under in vivo studies, 59% of the stem cuttings could be revived by the application of 0.1% CM-75 at the interval of 3 days and 39% of stem cutting by 0.2% of BV. CM-75 was found to be best for complete inhibition of in vitro fungal growth and in vivo revival of stem cuttings. Plant extracts of neem, nilgiri, mint, dhaniya, tulsi and turmeric were tested in vitro for antifungal activity, largest 40 mm zone of inhibition was recorded using tulsi and turmeric water extract. For complete inhibition of fungus, minimum inhibition concentration (MIC) of tulsi extract and turmeric extract was 20% and 30%, respectively. Neem, nilgiri, mint and dhaniya plant extracts were not active among tested concentration. This is the first report of callor rot disease management of stem cuttings using CM-75.