గ్లోబల్ జర్నల్ ఆఫ్ బయాలజీ, అగ్రికల్చర్ & హెల్త్ సైన్సెస్

నైరూప్య

IN VITRO STUDIES IN KYDIA CALICINA ROXB

Sanjay R. Biradar, Shrikant Bhosale, Somnath Kirwale, & Narayan Pandhure

Liver plays a very important role in the metabolism of foreign compounds entering the body. The exposure to the foreign compounds may be through consumption of alien/contaminated foods from exposure to chemical substances in the occupation environment or through synthetic drugs consumed for various pathological conditions; these compounds have many toxic effects on the human liver. The liver gets injured also by viruses, chemicals, alcohol and autoimmune diseases. Liver diseases remained one of the serious health problems, so medicinal plants and herbs have been used for treating such problems as in the Indian traditional systems of medicine, especially Ayurveda. Recently, a scientific basis was proved to justify the various medicinal uses of herbs like kydia calicina. Kydia calicina is an important medicinal plant used in treatment of skin diseases, curing arthritis and lumbago and relieving body pains. In the present investigation, efforts have been made to develop a suitable experimental protocol for regeneration and micropropagatrion of this important plant in vitro. Explants from mature plant of this species were collected and cultured on MS medium supplemented with various concentrations (0.5, 1.0 2.0 and 3.0 mg l-1) of cytokinins (BAP and Kn) and auxins (IAA, NAA and 2, 4-D) alone and in various combinations under controlled conditions. Successful regeneration method was achieved by this method.