జర్నల్ ఆఫ్ ఫార్మకోజెనోమిక్స్ & ఫార్మాకోప్రొటోమిక్స్

నైరూప్య

Fungi Grow Faster than Gram-Negative Bacteria in Emb Media and Also Deterring the Growth of These Bacteria by Reducing the pH of that Media

Imran Hossain, Sarafat Ali

Fungus (singular) are a kingdom that includes multicellular eukaryotic organisms such as yeast, mould etc. These are heterotrophs (cannot make their own food) but have significant roles in nutrient cycling. To obtain nutrients from organic material, they use their hyphae, which elongate and branch off swiftly, using the mycelium quickly they increase their size. Currently have a few number media these are suitable for fungus growth such as Sabouraud dextrose, malt extract and brain heart infusion medium, etc. Although we decide that fungi cannot suit them to grow in bacterial media but fungi can suit them in bacterial EMB media which act as a selective media to differentiate Gram-negative bacteria. Eosin Methylene Blue (EMB) known as "Levine's formulation” is the selective and differential medium for Gram-negative bacteria. In EMB media fungi even grow faster than gram-negative bacteria. Besides this faster growing of fungi, it deterring the growth of gram-negative bacteria by reducing the pH. The majority of the time, fungi requires specific conditions to flourish. Even in the scientific sector, we know that fungus does not grow well on bacterial media. But in this research, we observed the fungal growth specially mould (Aspergillus Niger) in EMB media along with its retardation activity of gram negative coliform bacterial growth. For this new finding assurance we performed the bacterial and fungal identification test further along with repeating the three times of whole experiment and we found the same result. The fungus was Aspergillus Niger and a bacterium was E. coli.