Osho Ciscars
The objective in formulating a SFM is to supplement a basal
medium such as DMEM/F12 with essential components such
as growth factors, vitamins, trace elements, hormones and
any other micronutrients not provided by the basal media.
Early attempts to develop SFM formulations incorporated such
animal-sourced components as insulin, transferrin, albumin and
cholesterol. However, these first-generation formulations still
had the disadvantages of containing relatively high protein
content and components that were derived from animal sources.
There followed two important, but separate, criteria for SFM:
protein-free (PF) and animal-derived component-free (ADCF).
ADCF media may contain recombinant proteins and protein
hydrolysates derived from non-animal sources