జర్నల్ ఆఫ్ ప్లాంట్ పాథాలజీ & మైక్రోబయాలజీ

నైరూప్య

Entomopathogenic Effect of Beauveria bassiana (Bals.) and Metarrhizium anisopliae (Metschn.) on Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)Larvae Under Laboratory and Glasshouse Conditions in Ethiopia

Tadele S and Emana G

Tomato leaf miner, Tuta absolute (Meyrick) is one of the major pest that infest tomato plant in all agro-ecological regions of the world where it present. Currently, the management strategies highly rely on chemical insecticides, which do not provide effective control and at the same time have environmental concern in addition to the residue left on the fruits. Hence, looking for alternative control measure is vital. Studies were conducted to determine the pathogenicity and virulence of three different concentrations of Beauveria bassiana and Metarhizium anisopliae against larvae of T. absoluta using the concentrations of 2.5 × 107, 2.5 × 108, and 2.5 × 109 conidia ml-1 under laboratory and glasshouse conditions. The experiments were carried out in the laboratory and glasshouse. Mortalities caused by B. bassiana isolate at the different concentrations ranged from 79.17% to 95.83% under laboratory and 73.0% to 84.04% under glasshouse, the highest mortality percentage being found at 2.5 × 109 conidia ml-1. The isolate of M. anisopliae caused the highest mortality also at the highest concentration. The lowest lethal time for B. bassiana and M. anisopliae , were achieved by the concentration 2.5 × 109 (5.01 days) and 2.5 × 108 (5.21 days), respectively. The isolates of B. bassiana and M. anisopliae, at 2.5 × 109 conidia ml-1 are promising for use the integrated control of T. absoluta larvae.