జర్నల్ ఆఫ్ ప్లాంట్ పాథాలజీ & మైక్రోబయాలజీ

నైరూప్య

Effect of Growth Media on the Pathogenicity of Enset Bacterial Wilt Pathogen ( Xanthomonas campestris pv. musacearum)

Misgana Mitiku, Alemar Said

The nutritional requirements of plant pathogenic bacteria vary with the bacterial species and even the bacterial isolates under consideration. Some media are considered general all-purpose media and support growth of a large variety of organisms. From previous work experience, disease/EBW (Enset Bacterial Wilt) development intensity of pure isolate is less compared to its fresh isolate. Chemical/nutrient composition of different media is relatively different. Some chemicals in the medium may affect the level of pathogenicity of Xcm. during its growth stage. Identification of appropriate media for Xcm pure culture isolation is important. Therefore the current research work done with the objective of identifying bacterial growth media that has effect on pathogenicity of Xcm (X. campestris). To do this the pathogenicity of the isolates has been evaluated by growing it on four different growth media (NA, NYA, YDC and one bulla based medium) and inoculated on to susceptible enset clone (Arkya) on pot experiment using Complete Randomized Design (CRD). Analysis of variance showed that among the tested growth media, there is no significant difference in pathogenicity of the bacterial wilt pathogen. So, we recommended that use of these growth media alternatively is possible at recommended rate of ingredients for pathogenicity and genotype screening experiment. But in terms of cost Nutrient Agar media (NA) is much cheaper than the rest of growth media so we recommended use it for isolation and identification work.