బాక్టీరియాలజీ & పారాసిటాలజీ జర్నల్

నైరూప్య

Antimicrobial Evaluation of Crude Methanolic Leaf Extracts from Selected Medicinal Plants Against Escherichia coli

Hibert Rachuonyo Opinde, Gatheri GW and Nyamache AK

This study was aimed at determining the antibacterial effects of the selected plant leaf extracts of Tagetes minuta, Aloe secundiflora, Vernonia lasiopus and Bulbine frutescens against clinical isolate of Escherichia coli. The plants materials were obtained from Kenyatta University arboretum and identified by University taxonomist and voucher specimen deposited in the University. Methanol was used as the solvent for the extraction process and the antimicrobial activity test carried out using the disc diffusion method. All the plants extracts analysed had antimicrobial activity against Escherichia coli with Tagetes minuta extract being the most active at low concentrations (8.7 mg/ml). The standard antibiotic used for positive control was ciprofloxacin (5 μg/ml) while distilled water and dimethyl sulphoxide were used as negative control. Screening of phytochemical showed the presence of four phytochemicals; saponins, tannins, alkaloids and flavonoids.