నానోమెడిసిన్ & నానోటెక్నాలజీ జర్నల్

నైరూప్య

Antibacterial Activity of Synthesized Silver Nanoparticles from Tinospora cordifolia against Multi Drug Resistant Strains of Pseudomonas aeruginosa Isolated from Burn Patients

Khushboo Singh, Manju Panghal, Sangeeta Kadyan, Uma Chaudhary and Jaya Parkash Yadav

In the present study, antibacterial activity of silver nanoparticles synthesized from stem of Tinospora cordifolia were analysed against multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn patients. As Pseudomonas aeruginosa is a scourge of hospital burn units and its emergence as multidrug-resistant strains is a major problem in the control of nosocomial infections. Therefore, we tried to establish a combination of medicinal values of Tinospora cordifolia and nanotechnology possibly with the field of medicine for the development of antibacterial agents against these MDR strains.The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, Energy Dispersive Spectroscopy and Fourier Transform Infrared Spectroscopy. Transmission Electron Microscopy and X-Ray Diffraction have revealed the size of silver nanoparticles 9 ± 36 nm and 12.49 nm respectively. Further antibacterial activity of silver nanoparticles prepared from Tinospora cordifolia against multidrug resistant strains was determined by agar well diffusion assay and Minimum Inhibitory Concentration (MIC) was estimated by qualitative experimentation by resazurin based micro broth dilution method. All experiments were done in triplicate. The silver nanoparticles of stem of Tinospora cordifolia showed the zone of inhibition ranges from 10 ± 0.58 to 21 ± 0.25mm. The MIC of AgNPs from stem extract was found to be 6.25 to 200 μg/ml against Pseudomonas aeruginosa strains. Silver nanoparticles from Tinospora cordifolia possess very good antibacterial activity which makes them a potent source of antibacterial agent.