నానోమెడిసిన్ & నానోటెక్నాలజీ జర్నల్

నైరూప్య

Therapeutic use of Fisetin and Fisetin Loaded on Mesoporous Carbon Nanoparticle (MCN) in Thioglycollate-induced Peritonitis

Shinjini Mitra, Silpak Biswas, Aryabaran Sinha, Nikhil Ranjan Jana and Ena Ray Banerjee

Background: The pathophysiology of aseptic peritonitis involves inflammation of the serosal membrane that lines the abdominal cavity and the organs contained therein. The etiology of peritonitis is complicated and is involved in various processes, of which, the most important one is the inflammatory reaction. During the pathological process of peritonitis, NF-κB plays an activating role in the inflammatory reaction, which might be a potential therapeutic target in the therapy of certain inflammatory diseases. We studied the anti-inflammatory and pro-regenerative actions of Fisetin, a flavonol found in many plants, in a mouse model of thioglycollate-induced peritonitis, as well as the actions of fisetin administered with a nanoparticle such as mesoporous carbon nanoparticle (MCN). BALB/c mice were used in this study. Results: We found cell recruitment in the blood increased with the administration of thioglycollate (TG) after 24 h, 48 h, 72 h and 96 h, showing that it has induced inflammation. Cell recruitment was successfully inhibited by fisetin, and with MCN+fisetin. In the peritoneal fluid, total cell recruitment was increased, which was successfully inhibited with fisetin and MCN+fisetin treatment. TG treatment significantly reduced cell proliferation in the blood, PF and BM, within 24 h, till 96 h. Interestingly, cell proliferation increased with fisetin treatment, and with MCN+fisetin. The clonogenic potential of the tissues decreased significantly within 24 h, with administration of TG. Both fisetin treatment and MCN+fisetin treatment restored the clonogenic potential of the tissues. There was a decrease in Th2 cytokines with TG treatment, in blood after 48 h, and both fisetin and MCN+fisetin increased the cytokine content. Conclusion: In conclusion, we found that fisetin had a promising therapeutic effect on the peritonitis.