జర్నల్ ఆఫ్ ఆక్వాకల్చర్ రీసెర్చ్ & డెవలప్‌మెంట్

నైరూప్య

The Effectiveness of Silver Nanoparticles for the Treatment of Abdominal Dropsy in Labeo rohita

Maryyum Khalil, Summia Perveen, Hafiz Muhammad Arslan-Amin, Iqra Anwar, Mahrukh Butt

Despite using antibiotics, in aquaculture of freshwater sp. facing severe challenges of bacterial strain resistance occur. In order to combat disease-causing bacterial species, the present study was designed to assess the value of green synthesized silver nanoparticles (Ag NP´s) from the extract of plant leaves for abdominal dropsy treatment in Labeo rohita. For this purpose, an extract of lemon leaves extracts in powder form was prepared followed by a dry method and a mixture of ethanol and distilled water (4:6) was added. Prepared nano-solution was then subjected to further characterization of particles where color changes, UV-VIS spectroscopy, particle analysis, and FTIR were done. Statistical results showed maximum (SGR%) value (2.65 ± 0.010) in T1 nano solutions treated group, had a significant difference (P<0.05) as respect to other treatments and control groups. Water physicochemical properties showed a significant result (P <0.05). At the end of the trial, total protein, albumin, globulin, glucose, ALT, and AST were analyzed from serum samples of both (diseased & healthy fish) and showed significant results (p <0.05) compared to other treatment groups. By histopathology detection, no hepatocyte damage, vacuolization and, regular shape nuclei were detected in healthy fish, also fibrosis and coagulative necrosis were absent, while in diseased fish, damaged hepatocytes were detected with irregular shaped nuclei and vacuolization. Thus, our study suggests that the lemon plant leaves extract solution could be very effective for the treatment of bacterial disease in Labeo rohita.s.