గ్లోబల్ జర్నల్ ఆఫ్ బయాలజీ, అగ్రికల్చర్ & హెల్త్ సైన్సెస్

నైరూప్య

IN VITRO SHOOT GENERATION FROM COTYLEDON EXPLANT OF BRASSICA OLERACEA USING KIN AND NAA

Nazirwan

Cotyledon were used as explants in in vitro plant regeneration of broccoli (Brassica oleracea). The objective of this research was to examine the influence of the growth regulators 6- Furfuryl amino purine (KIN) and naphthalene acetic acid (NAA) on shoot formation in these cultivars. The shoot regeneration system of cotyledoned explants can be useful as a tool for synthetic seed formation. Cotyledon explants excised from 14- day-old in vitro germinated seedlings were placed on shoot induction medium containing basal salts of Murashige and Skoog (MS) and various concentrations of KIN and NAA. The highest percentage of cotyledon explant producing shoot (70%) and the highest mean number of shoots produced per cotyledon explant (1.80) were obtained on 2 mg/l KIN with 0.5 mg/l NAA. Therefore, 2 mg/l KIN with 0.5 mg/l NAA are the recommended combinations for shoot regeneration from cotyledonary explants.