ఫార్మాస్యూటికా అనలిటికా ఆక్టా

నైరూప్య

Determination of Nicotine Exposure Using Passive Sampler and High Performance Liquid Chromatography

Amagai T, Bai H, Wang Q, Miyake Y, Noguchi M and Nakai S

To measure nicotine personal exposure in our daily life, we have developed a determination method of personal exposure to nicotine. The method consisted of passive sampling of nicotine, simple solvent extraction and high performance liquid chromatography (HPLC) determination. The passive sampler was badge type and collection media was sodium bisulfate impregnated quartz fiber filter. The collected nicotine as nicotine bisulfate was extracted with pure water simply. Nicotine bisulfate was separated by HPLC, and detected with spectrophotometry. Three kinds of HPLC separation column were tested, and the best separation was attained with a cation exchange column, Zorbax 300-SCX. We have also evaluated the kinds of filter used, stability of nicotine bisulfate. Nicotine bisulfate on the filter was not evaporated or degraded in the outdoor air. The amount of collected nicotine was converted to air concentration by the equation that was made from simultaneous active- and passive- sampling experiments. Then it was suggested that nicotine determination method developed in this study was useful for estimate nicotine personal exposure accurately.