జర్నల్ ఆఫ్ ప్లాంట్ పాథాలజీ & మైక్రోబయాలజీ

నైరూప్య

Assessment of the Inhibitory Activity of Resin from Juniperus procera against the Mycilium of Pyrofomes demidoffi

Dagnew Bitew

Juniperus procera is an evergreen dioecious more seldom monoecius tree, which belongs to the family Cupressaceae and the only Juniper species, which is found in the mountains of East Africa and it is an important indigenous forest tree species in Ethiopia. However J. procera is subjected to a serious attack by the slow growing white heart-rot fungus, Pyrofomes demidoffii. Resin has been reported to be active against wood colonizing fungi, however, the role of resin of J. procera in protecting the tree from the attack of P. demidoffii is not known. Therefore this study has initiated to assess the inhibitory effect of resin from J.procera against a white rot fungi P. demidoffii. Resin and basidiocarps of P. demidoffii was collected from infected J. procera trees at Menagesha Suba Forest situated 30 km South West of Addis Ababa, Ethiopia. The antifungal activity of J. procera resin against P. demidoffii was tested using agar dilution assay technique and an impressive result was observed. The MIC value of resin extract was within the range of 5 to 6 mg/100 ml MEAP. Phytochemical screening test for resin extract was done and it revealed the presence of alkaloids, saponins, terpenoids, phenolic compounds, flavenoides and fixed oils and the absence of carbohydrate, glycoside, steroids and fats. The crude extract of J. procera resin was fractionated into six fractions with column chromatography using different organic solvents and all fractions run on Thin Layer Chromatography (TLC) plate using benzene: methanol (18:6) and benzene: ethanol: ammonia (18:2:1). All fractions gave different retention factor (Rf) in each developing solvents.