జర్నల్ ఆఫ్ ప్లాంట్ పాథాలజీ & మైక్రోబయాలజీ

నైరూప్య

Antifungal Evaluation and Phytochemical Identification of Selected Botanicals against Ceratocystis manginecans Causing Mango Sudden Death

Kokab Jabeen, Shahzad Asad and Muhammad Zakria

The antifungal efficacy of three selected plants (D. viscosa, Citrullus colocynthis, and Ailanthus altissima) was evaluated against Ceratocystis manginecans (causal agent of Mango Sudden Death) using poisoned food technique. Ethanol, methanol and aqueous extracts of selected plants prepared at 2.5 g/100 ml, 05 g/100 ml and 10 g/100 ml concentration were evaluated against Ceratocystis manginecans. Most effective botanical extracts were screened for their phytochemical constituents through GCMS. Results revealed that ethanol crude extract of D. viscosa exhibited highest antifungal efficacy (85.066%) followed by Citrullus colocynthis (82.664%) and Ailanthus altissima (69.112%). All botanicals exhibited statistically significant antifungal efficacy from each other (p<0.05). The ethanol extracts were most effective than methanol and water extracts of selected plants against Ceratocystis manginecans. Botanical treatments resulted in thin, collapsed/damaged hyphae as compared to control. Phytochemical profiling of most effective botanical extracts revealed that 9-Octadecanoic acid and I-(+)- Ascorbic acid 2,6-Hexadecanoate were found common in all three most effective botanical extracts. The present study revealed that these compounds possibly contributed to the antifungal efficacy of botanicals against C. manginecans.