జర్నల్ ఆఫ్ ప్లాంట్ పాథాలజీ & మైక్రోబయాలజీ

నైరూప్య

Antibacterial and Antifungal Activity in Leaf, Seed Extract and Seed Oil of Seabuckthorn (Hippophae salicifolia D. Don) Plant

Sanjay Mohan Gupta, Atul K Gupta, Zakwan Ahmed and Anil Kumar

The antibacterial and antifungal efficacy of crude leaf and seed extract and seed oil of Seabuckthorn (Hippophae salicifolia D. Don) on different microbial cultures causing infections/diseases was investigated by agar disc diffusion method. Kanamycin (1000 ?g/ml) and clotrimazole (100 ?g/ml) was used as standards drug for antibacterial and antifungal assay, respectively. The inhibition zones ranged from 7 to 23 mm for antibacterial and from 10 to 27 mm for antifungal activity, respectively. Among all extracts tested, seed extract showed significant and leaf extract showed intermediate activity against both gram-positive bacteria. Whereas, no activity was observed against all test extracts in gram-negative bacteria except in case of Agrobacterium tumefaciens some activity was obtained against seed extract of H. salicifolia. In case of fungal cultures, among all test extracts only seed extract showed significant activity against Mucor and Tilletia fungus, while in case of Rhizopus no activity was observed against all test extracts. These results indicated the possibility of using H. salicifolia extract and seed oil for medicinal uses and natural food preservation.